Master 1 / Master 2 Internship : Nuclear architecture and melanoma cell plasticity

id="offer-description" class="ecl-u-type-heading-2 ecl-u-mt-none ecl-u-mb-l">Offer Description Context . Melanoma is a highly heterogeneous cancer characterized by a remarkable capacity to adapt to environmental and therapeutic constraints. Beyond genetic alterations, cellular architecture, nuclear organization and physical forces are increasingly recognized as important contributors to tumour cell behaviour and plasticity. Internship project . The aim of this internship is to investigate how changes in nuclear morphology, nuclear organization and cellular mechanics are associated with melanoma cell plasticity and adaptation. The student will work in an interdisciplinary research environment combining cancer biology, molecular and cellular biology, fluorescence microscopy and quantitative approaches. Techniques and training . Depending on the progress of the project, the student will have the opportunity to gain experience in:. melanoma cell culture and experimental cell biology,. siRNA/shRNA-based gene modulation,. real-time proliferation and viability assays,. migration and invasion assays,. fluorescence and confocal microscopy,. immunofluorescence,. quantitative analysis of cell and nuclear morphology,. RNA extraction, RT-qPCR and Western blotting,. chromatin-based approaches,. quantitative image and data analysis. Candidate profile . We are looking for a motivated Master 1 or Master 2 student with a background in cell biology, molecular biology, cancer biology or related