Post-doctorant (M/F), in the field of RNA and translation

id="offer-description" class="ecl-u-type-heading-2 ecl-u-mt-none ecl-u-mb-l">Offer Description The postdoctoral researcher will take part in the ERC Synergy project 3Stops2go, which brings together four teams around the engineering of alternative decoding for therapeutic purposes. The project developed at I2BC pursues two complementary and clearly identifiable objectives. The first is to design, implement and analyse a large-scale screen in human cells in order to identify novel suppressor tRNAs able to read through premature termination codons (PTCs) with high efficiency and, above all, with good selectivity towards native stop codons. The second is to elucidate the mode of action of TLN468 and its derivatives, a readthrough inducer identified by the team, more efficient than gentamicin and endowed with an unusual selectivity regarding the amino acid incorporated at each stop codon, but whose precise molecular mechanism remains to be established. The successful candidate will contribute to key deliverables of the project, including the design of quantitative reporters, the implementation of functional screens, the analysis of high-throughput data (NGS, ribosome profiling) and the mechanistic interpretation of the results. The activities carried out in the course of this project will be:. - Molecular biology approaches (cloning, expression, in vitro transcription and translation). - Biochemical approaches (protein purification, luciferase assays). - Human cell culture, handli