CDD Post-doc in yeast evolutionary genomics (M/F)

id="offer-description" class="ecl-u-type-heading-2 ecl-u-mt-none ecl-u-mb-l">Offer Description The post-doc will contribute to the ANR project EDGES (Evolving genomes beyond species boundaries), which investigates how genomes acquire, assimilate and evolve large amounts of foreign and extra-self DNA (fsDNA) in the budding yeast Saccharomyces cerevisiae. The post-doc will lead work on the natural uptake of environmental DNA, the reprogramming of mating to enable interspecies hybridisation, and the adaptive potential of fsDNA through experimental evolution. The post-doc will also collaborate on other projects currently ongoing in the group ( https://ircan.org/en/research/gianni-liti ). The post-doc will perform both experimental and computational analyses to determine how foreign DNA enters yeast cells, how it is maintained, and what its phenotypic and evolutionary consequences are. Main tasks:. * Design and use of a synthetic mini linear yeast artificial chromosome with a fluorescent reporter to measure natural DNA uptake across genetic backgrounds and ecologically relevant conditions, including cell sorting, transcriptomics and GWAS-based mapping of the genetic determinants of natural competence. * Engineering of a synthetic mating system to overcome prezygotic barriers and enable hybridisation and cytoduction between S. cerevisiae and distant non-Saccharomyces species, monitored at the single-cell level by fluorescence and time-lapse microscopy. * Adaptive laboratory evoluti