Bioengineering of regionalized human cortical models on a chip (M/F)

id="offer-description" class="ecl-u-type-heading-2 ecl-u-mt-none ecl-u-mb-l">Offer Description We offer a highly interdisciplinary, inclusive and international work environment at the Cell and Cancer Physics Unit (CNRS, Institut Curie, PSL, SU). Located in the heart of the rich Latin Quarter in Paris, our unit hosts experimental and theoretical physicists, biologists and biochemists. We are deeply committed to fostering diversity and are looking for talented scientists in all fields at the physics-biology interface. Brain organoids are major models of human brain development, but they still imperfectly reproduce the regionalisation and layering of the cortex. The ERC Synergy PatCorg project aims to create organoids that more closely recapitulate the organisation of the human cortex, using microfluidics. The objective of the doctoral project will be to develop a cortex-on-chip model to spatially and temporally control the delivery of morphogens in order to induce cortical organizing centers and to study the formation of regionalization axes comparable to the anterior-posterior and medio-lateral axes of the developing cortex. Activities, Main missions. Culture and differentiation of human pluripotent stem cells into neuroepithelium and cortical organoids. Use and optimization of μ-transwell microfluidic devices to locally deliver morphogens on the basal surface of the tissue. Study of the influence of concentration, duration of exposure, combinations of morphogens and distance