Researcher in Electrophysiology and Neurobiology of Pain (M/F)

id="offer-description" class="ecl-u-type-heading-2 ecl-u-mt-none ecl-u-mb-l">Offer Description -The successful candidate will be recruited as part of the ANR project "PROTECT-DN". Their responsibilities will include:. Characterizing the excitability of murine sensory neurons in a model of type 2 diabetic neuropathic pain (induced by a high-fat, high-sugar diet) compared to control animals, by comparing genetic inhibition models of the Cav3.2 calcium channel. Methodological approaches will include ex vivo electrophysiology of sensory fibers and neurons (skin-nerve preparations and patch-clamp recordings), as well as in vivo calcium imaging or extracellular electrophysiology in anesthetized mice (miniscope videofluorimetry or single/multi-unit action potential recordings). Characterizing the excitability of murine sensory neurons in the same type 2 diabetic neuropathic pain model, comparing animals chronically treated with a T-type calcium channel pharmacological inhibitor currently in clinical development. The methodological approaches will be identical to those outlined in Point 1. Conducting longitudinal tracking of mechanical and thermal pain sensitivity thresholds in cohorts of mice subjected to the type 2 diabetes model, utilizing a novel automated behavioral analysis platform (RAMalgo system) ensuring unbiased assessment. A development phase will consist of coupling this automated pain evaluation with electrophysiological or videofluorimetric recordings. Participating in